Peer-reviewed veterinary case report
Pseudomonas aeruginosa types causing eye infections in dogs
By Ledbetter, Eric C et al.·Published in Investigative ophthalmology & visual science·2009·College of Veterinary Medicine, United States·View original on PubMed →
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Original publication title: Pathogenic phenotype and genotype of Pseudomonas aeruginosa isolates from spontaneous canine ocular infections.
- Species:
- dog
Plain-English summary
A group of dogs with eye infections, specifically keratitis (inflammation of the cornea), were studied to understand the types of Pseudomonas aeruginosa bacteria causing these issues. The researchers found that certain strains of this bacteria were more likely to invade and harm the cells in the cornea, particularly in dogs with active keratitis. Older dogs were more affected by invasive strains, while younger dogs had strains that were more cytotoxic. The study highlighted that the bacteria causing keratitis had a unique ability to damage corneal cells without causing immediate cell death.
People also search for: dog eye infection treatment · Pseudomonas aeruginosa in dogs · keratitis in dogs symptoms
Abstract
PURPOSE: This study was designed to determine whether the ability to adversely affect corneal epithelial cell health is a factor common to Pseudomonas aeruginosa keratitis strains and to assess the prevalence of each pathogenic phenotype and genotype in a canine model of naturally-acquired P. aeruginosa ocular infection. METHODS: P. aeruginosa ocular isolates were collected by sampling 100 dogs without disease (six isolates collected) and by sampling dogs with conjunctivitis (two isolates), endophthalmitis (one isolate), active keratitis (12 isolates), and resolved P. aeruginosa keratitis (four isolates). Phenotype was determined in vitro by quantifying corneal epithelial cell invasion by gentamicin survival assays, and cytotoxic activity by Trypan blue exclusion assays. Genotyping was performed for genes encoding the type III secreted effectors. RESULTS: The ratio of invasive to cytotoxic strains with 95% confidence intervals (CI) was 0.83 (CI, 0.42-0.99) for conjunctival microflora isolates, 0.80 (CI, 0.54-0.94) for ocular infection isolates, and 1.0 (CI, 0.45-1.0) for strains isolated post-resolution of keratitis. Among ocular infection isolates, invasive and cytotoxic strains were significantly (P <or= 0.02) associated with older and younger dogs, respectively. Visible adverse effects on epithelial cells were significantly (P <or= 0.03) more frequent for keratitis strains (6/12) than other strains (1/13), but only three of these keratitis strains and the single non-keratitis strain possessed ExoU. CONCLUSIONS: Invasive strains predominated in the dogs of this study. Only keratitis strains had visible adverse effects on epithelial cells without overt cytotoxicity, suggesting virulence strategies affecting live corneal epithelial cell health are selected for among keratitis strains.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/18836164/