Peer-reviewed veterinary case report
Rapid in-clinic test accurately detects canine pancreatic lipase
By Beall, Melissa J et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2011·IDEXX Laboratories Inc., United States·View original on PubMed →
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Original publication title: Performance validation and method comparison of an in-clinic enzyme-linked immunosorbent assay for the detection of canine pancreatic lipase.
- Species:
- dog
Plain-English summary
A dog with acute vomiting, loss of appetite, and abdominal pain may be suffering from pancreatitis, a condition that's often tricky to diagnose. A new in-clinic test for measuring a specific enzyme related to pancreatic health can provide quick results, helping vets assess the situation right away. This test showed a high agreement with traditional lab tests, meaning it can be trusted to help diagnose pancreatitis effectively. Using this rapid test alongside standard lab tests can give a clearer picture of the dog's condition and lead to timely treatment.
People also search for: dog vomiting pancreatitis treatment · how to diagnose pancreatitis in dogs · canine pancreatic lipase test results
Abstract
Diagnosis of pancreatitis is often difficult in dogs that present with acute vomiting, anorexia, and abdominal pain, as these clinical signs may occur with a variety of other illnesses. While quantitative reference laboratory methods specific for canine pancreatic lipase are available to aid in diagnosis, results are generally not available until the next day. The objective of the current study was to validate a semiquantitative in-clinic rapid test for the measurement of canine pancreas-specific lipase (cPL) and to compare its performance to the reference lab method. Comparison of the reference method for cPL to the in-clinic assay demonstrated 96-100% agreement for canine serum samples with normal levels of cPL and 88-92% agreement for samples with elevated levels of cPL. Common interfering substances such as bilirubin, lipids, or hemoglobin had no effect on assay performance. Both within-day and day-to-day variations ranged from 10% to 20% of the calculated cPL concentration, which demonstrated a high degree of precision for the in-clinic assay. Performance of 3 lots of the in-clinic assay with the same set of canine serum samples demonstrated high assay reproducibility, with interclass correlation coefficients of ≥0.93. Results of the in-clinic cPL assay, based on both visual and calculated cPL concentrations, were consistent throughout 15 months of storage. The in-clinic test provides immediate, semiquantitative results to supplement existing pancreatitis diagnostics at the time of acute illness. Because the reference and in-clinic methods are aligned, they can be used together as an immediate aid pet-side and as a fully quantitative follow-up test at the reference laboratory.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/21217040/