Peer-reviewed veterinary case report
Peroxidase-linked assay for detection of antibodies against bovine leukosis virus.
- Journal:
- Journal of immunoassay & immunochemistry
- Year:
- 2013
- Authors:
- de Castro, Clarissa C et al.
- Affiliation:
- Laboratory of Virology and Immunology · Brazil
Abstract
A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23859788/