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Peer-reviewed veterinary case report

Persistence of Bluetongue virus serotype 1 virulence in sheep blood refrigerated for 10 years.

Journal:
Veterinaria italiana
Year:
2018
Authors:
Puggioni, Giantonella et al.
Affiliation:
Istituto Zooprofilattico Sperimentale della Sardegna · Italy

Abstract

This paper reports that Bluetongue virus serotype 1 (BTV-1) infected blood collected during the 2006 Sardinia (Italy) epidemic from a ewe with clinical disease and stored at ~ 5°C for 10 years, caused Bluetongue (BT)-like clinical disease and death when inoculated into a susceptible Sarda breed ram. Anatomo-histopathological examination and Real-Time Reverse Transcriptase PCR (Real-Time RT-PCR) confirmed the presence of BTV-1 in several tissues proving that the BTV-1 2006 isolate has maintained its infectivity and virulence.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/30681135/