Phenol-soluble modulin alpha (PSMα) mediates autophagy induction and intracellular survival ofin bovine mammary epithelial cells.

Abstract

() is a Gram-positive bacterium that serves as a major pathogen causing bovine mastitis. One of the key intracellular survival mechanisms ofinvolves the induction of autophagy. The capacity ofto subvert autophagy for intracellular survival and replication is strongly linked to its broad array of virulence determinants, which play pivotal roles in infection establishment and immune evasion. One of these virulence factors is phenol-soluble modulin alpha (PSMα), an amphipathic small peptide that plays a key role in the pathogenesis of. Utilizing gene-editedstrain and bovine mammary epithelial cells (BMECs, MAC-T), this study elucidates the involvement of PSMα in autophagy induction duringinfection. The results demonstrated that PSMα knockout attenuated-induced autophagy, weakened the activation of the PI3K/Akt/mTOR pathway, and reduced intracellular bacterial load. Our findings elucidate a PSMα-dependent autophagy induction mechanism ininfection, which is associated with the intracellular survival of.