Peer-reviewed veterinary case report
Phenotypic High-Throughput Screening and Drug Efficacy Test Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2026
- Authors:
- Wang, Chenchen et al.
- Affiliation:
- Institute of Zoonosis · China
Abstract
An effective method to quantify parasite loads is key to evaluating anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening of drugs against Cryptosporidium parvum was previously infeasible using labor-intensive traditional assays. Here, we describe a simplified quantitative RT-PCR assay that directly uses cell lysates as templates, enabling high-throughput quantification of parasite loads. This assay can also be used to evaluate drug efficacy against the growth of C. parvum in vitro.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/41144213/