Phylogenetic analysis ofisolated from broilers with colibacillosis based ongene sequences.

Abstract

isolates from chickens with colibacillosis were assigned to phylogenetic groups based on multiplex polymerase chain reaction (PCR) and antibacterial resistance ofbelonging to these groups was examined. Furthermore, theA gene of isolates was sequenced and a phylogenetic tree was generated. A total of 84isolates were grouped using multiplex PCR of TSPE4.C2,A,A, andA molecular markers. Four phylogenetic groups were identified with strains divided as follows: 16 in group A (19.05%), 17 in group B1 (20.24%), 23 in group B2 (27.38%), and 28 in group D (33.33%).isolates belonging to phylogenetic groups B2 and D were resistant to Soltrim and Flumequine unlike the majority ofisolates that belonged to groups A and B1, and which were susceptible to these antibiotics. The phylogenetic results based ongene sequences from multiplex PCR revealed thatphylogenetic grouping was in accordance with the clusters obtained in the phylogenetic tree. In conclusion, the comparative sequence analysis ofsequences provides a firm framework for an accurate classification ofand related taxa and may constitute a pertinent phylogenetic marker for