Physicochemical Characterization of <i>Camellia oleifera</i> Husks from Different Regions and Microwave-Assisted RSM Optimization of Tea Saponin Extraction.

Abstract

This study investigated the physicochemical properties of <i>Camellia oleifera</i> husks collected from three regions of Jiangxi Province (Ganzhou-GZ, Yichun-YC, and Jiujiang-JJ) and extracted tea saponins via microwave-assisted solvent extraction (MASE), aiming to provide a theoretical basis for the high-value utilization of this agricultural by-product. The husks from YC were rich in bioactive compounds such as tea saponins (16.29 ± 0.02%), with lower cellulose (21.05 ± 1.05%) and lignin (12.48 ± 1.14%) contents and higher hemicellulose (27.40 ± 0.80%) content. The husks from JJ exhibited abundant porosity and a larger specific surface area (40-60 mesh, 4.15 ± 0.04 m²/g). Single-factor extraction experiments indicated that the microstructure and chemical composition of <i>Camellia oleifera</i> husks significantly affected the extraction efficiency of saponins, tannins, and flavonoids. The optimal extraction conditions for tea saponins were established using Box-Behnken response surface methodology, with the liquid-to-solid ratio identified as the most critical factor. Optimal conditions for GZ husks were a liquid-to-solid ratio of 46.75 mL/g, ethanol concentration of 35.5%, extraction time of 6 min, and microwave power of 350 W, with the extraction yield of 7.49 ± 0.01%. Optimal conditions for YC husks were a liquid-to-solid ratio of 50.55 mL/g, ethanol concentration of 40.13%, extraction time of 6 min, and microwave power of 350 W, with the extraction yield of 16.29 ± 0.02%. Optimal conditions for JJ husks were a liquid-to-solid ratio of 47.44 mL/g, ethanol concentration of 37.28%, extraction time of 6 min, and microwave power of 350 W, with the extraction yield of 9.39 ± 0.02%. The study provides important scientific evidence for understanding the structure-function relationship of <i>Camellia oleifera</i> husks and offers practical guidance for developing sustainable industrial processes to convert agricultural by-products into high-value bioactive compounds, thereby promoting resource recycling and economic benefits in the <i>Camellia oleifera</i> industry.