Peer-reviewed veterinary case report
High levels of miR-21-5p in blood vesicles of dogs with skin mast
By Zamboni, Clarissa et al.·Published in Frontiers in veterinary science·2022·Dipartimento di Medicina Veterinaria e Scienze Animali, Italy·View original on PubMed →
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Original publication title: Plasma small extracellular vesicles from dogs affected by cutaneous mast cell tumors deliver high levels of miR-21-5p.
- Species:
- dog
Plain-English summary
A group of dogs with skin tumors called mast cell tumors (MCT) were studied to see if certain tiny particles in their blood, known as small extracellular vesicles (sEV), could provide information about their condition. Researchers found that dogs with more advanced tumors that had spread to lymph nodes had higher levels of a specific microRNA (miR-21-5p) in these particles compared to healthy dogs and those without lymph node involvement. This suggests that measuring these particles could help veterinarians understand the severity of the disease in dogs with MCT.
People also search for: dog mast cell tumor treatment · dog skin tumor symptoms · high levels of miR-21-5p in dogs
Abstract
Small extracellular vesicles (sEV) are a class of extracellular vesicles (30-150 nm), delivering molecules including proteins, metabolites, and microRNAs (miRNAs), involved in physiological intercellular crosstalk and disease pathogenesis. The present pilot study aims are (I) to develop an easy and fast protocol for the isolation of sEV from plasma of mast cell tumor (MCT)-affected dogs; (II) to evaluate if miR-21-5p (sEV-miR-21-5p), a miRNA overexpressed by MCT, is associated with sEV. Seventeen dogs have been enrolled in the study: 4 healthy and 13 (6 with and 7 without nodal metastasis) MCT-affected dogs. sEV were isolated using size exclusion chromatography (SEC) (IZON column 35nm) and were characterized by Western blot, Nanoparticle tracking analysis, and transmission electron microscopy. sEV-miR-21-5p was quantified using digital PCR. sEV expressed the specific markers CD9 and TSG101, and a marker of mast cell tryptase. The sEV mean concentration and size were 2.68E + 10 particles/ml, and 99.6 nm, 2.89E + 10 particles/ml and 101.7 nm, and 3.21E + 10 particles/ml and 124 nm in non-metastatic, nodal metastatic, and healthy samples, respectively. The comparative analysis demonstrated that the level of sEV-miR-21-5p was significantly higher in dogs with nodal metastasis compared to healthy (= 0.038) and without nodal metastasis samples (= 0.007). In conclusion, the present work demonstrated that a pure population of sEV can be isolated from the plasma of MCT-affected dogs using the SEC approach and that the level of sEV-miR-21-5p is higher in nodal metastatic MCT-affected dogs compared with healthy and MCT-affected dogs without nodal involvement.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/36704706/