Pleistophora infestation in fathead minnows, Pimephales promelas (Rafinesque).

Abstract

Pleistophora infestation was observed in adult fathead minnows, Pimephales promelas, held under laboratory conditions. Fish were clinically healthy, and presented no gross findings at necropsy. Histopathology revealed parasitic stages only in the ovaries. Spores within sporophorous vesicles were mainly encountered in late vitellogenic oocytes and were ultrastructurally identified as a microsporidian parasite. Heavily parasitized oocytes underwent degeneration followed by the release of spores into the ovarian interstitium. Degenerating oocytes and interstitial spores caused ovarian inflammation. Male fish showed no parasites in the testes. Parasitic infestation was compared with body length, body weight, gonadal weight, gonadosomatic index and plasma vitellogenin levels, and revealed no statistically significant differences between non-parasitized and parasitized females. The isolated holding conditions of the fish and the presence of parasitic stages in the ovaries suggested that an infestation with Pleistophora ovariaeSummerfelt, 1964 was more probable than that with Pleistophora mirandellae (Vaney & Conte, 1901).