Pluripotent epigenetic regulator OBP-801 attenuates fibrosis and maintains lower intraocular pressure in a rabbit PRESERFLO MicroShunt surgery model.

Abstract

The inhibition of fibrosis in filtering blebs is indispensable for lowering intraocular pressure (IOP) after PRESERFLO MicroShunt surgery (PMS). We previously reported the anti-fibrotic effect of OBP-801 (OBP) on a filtering bleb in a rabbit glaucoma filtration surgery model using a 22-gauge cannula. In this study, we investigated the effect of OBP after PMS in rabbit eyes for the development of a new practically applicable anti-fibrotic treatment. This study involved 19 Japanese white rabbits that underwent PMS in the right eye, with those eyes divided into 3 groups: (1) intraoperative subconjunctival injection of 0.02% mitomycin-C (MMC) (n&#x2009;=&#x2009;6), (2) postoperative instillation of 100&#xa0;nM OBP eye drops (n&#x2009;=&#x2009;7), or (3) a balanced salt solution (BSS) control (n&#x2009;=&#x2009;6). Bleb morphology and IOP were monitored for 12&#xa0;weeks postoperative, with the bleb tissues then undergoing evaluation of fibrosis and Western blot analysis. A lower postoperative IOP was maintained in the OBP-group eyes, and at 12&#xa0;weeks postoperative, the IOP was significantly lower in that group than in the BSS and MMC groups (p&#x2009;<&#x2009;0.01). OBP-treated eyes showed no adverse effects and reduced levels of alpha-smooth muscle actin and collagen deposition, thus suggesting that OBP is a promising candidate for improving surgical outcomes post PMS.