Polyhydrated ionogen with MgBr2 accelerates in vitro respiratory epithelial healing.

Abstract

BACKGROUND: Remucoslization of the sinonasal cavity after sinus surgery is critical for successful outcomes. Recently, a novel antiprotease and antifibroblast compound, polyhydrated ionogen (PHI) with MgBr2, showed improved wound healing in a rabbit maxillary sinus mucosal wound model. We set out to determine if this effect was reproducible in an in vitro respiratory epithelial culture system. METHODS: Fully differentiated mature murine nasal septal air-liquid interface cultures were injured by creating a full-thickness 400-mM-wide scratch through the monolayer. Cultures were then treated with nothing, saline, or PHI with MgBr2 for 1 hour on the apical surface. Twenty-four hours after the injury cultures were fixed and processed for immunofluorescence with type IV beta-tubulin and Hoechst stain. RESULTS: Initial injury resulted in a wound of 394 micromolar (377-411 micromoler; 95% CI; n = 8). After 24 hours with no intervention the wound closed to 161 micromolar (138-184 micromolar; 95% CI; n = 9) and treatment with saline resulted in a residual gap of 88 micromolar (60-116 micromolar; 95% CI; n = 9; p < 0.05) and treatment with PHI with MgBr2 resulted in a gap of only 30 micromolar (14-46 micromolar; 95% CI; n = 9; p < 0.05). CONCLUSION: Poor healing of the sinonasal mucosa after surgery with loss of ciliary function results in adverse clinical outcomes. In an in vitro sinonasal respiratory epithelial injury model, a one-time treatment with PHI with MgBr2 showed significantly improved wound healing compared with saline or nothing. This is a viable model to further investigate the mechanism by which PHI with MgBr2 improves sinonasal remucosolization.