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Peer-reviewed veterinary case report

Canine parvovirus in Bareilly dogs detected by PCR testing

By Jobin Thomas et al.·Published in Veterinary World·2014·Department of Immunology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India; jobss2k6@gmail.com, NZ·View original on DOAJ

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Original publication title: Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Species:
dog

Plain-English summary

A group of dogs in the Bareilly region were tested for canine parvovirus (CPV-2) after showing symptoms like vomiting and diarrhea. Out of 44 fecal samples collected, 23 tested positive, with most cases found in male Labrador puppies aged 3 to 6 months. Interestingly, about 17% of the vaccinated dogs also tested positive. This suggests that while vaccination helps, it may not completely prevent the disease. Further testing is needed to confirm these findings and understand the virus strains in the area.

People also search for: dog vomiting diarrhea · parvovirus in puppies · Labrador puppy vaccination effectiveness

Abstract

Aim: The aim of this study was to screen the suspected samples by polymerase chain reaction (PCR) and epidemiological analysis of positive cases of canine parvovirus type2. Materials and Methods: Fecal samples were collected from dogs suspected for canine parvovirus type 2 (CPV-2) and viral DNA was extracted. Primers were designed, and PCR was done with all extracted DNA samples. Age, sex and breed wise distribution of positive cases were analyzed. Results: Out of a total 44 collected fecal samples, 23 were found to be positive for CPV-2 by developed PCR. The disease was found to be more common in Labrador male pups of 3-6 months of age. The percentage of positive cases in vaccinated dogs was found to be around 17.4%. Conclusion: Almost half (52.3%) of total collected samples were found to be positive by PCR. However, number of field samples are needed to further validate this test and additionally sequence analysis needs to be done to ensure the prevalent field strain of CPV-2.

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Original publication on DOAJ: https://doi.org/10.14202/vetworld.2014.929-932