Peer-reviewed veterinary case report
Fast PCR test with magnetic cleanup detects canine parvovirus 2
By Zhuang, Linlin et al.·Published in BMC veterinary research·2019·School of Biological Sciences and Medical Engineering and Collaborative Innovation Center of Suzhou Nano Science and Technology, China·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2.
- Species:
- dog
Plain-English summary
A puppy with severe vomiting and diarrhea was tested for canine parvovirus (CPV-2), a common virus that causes gastroenteritis in dogs. Researchers developed a new testing method that combines polymerase chain reaction (PCR) with a quick lateral flow test to detect the virus more efficiently. This method was able to accurately identify CPV-2 in samples and provided results in about 80 minutes. This rapid detection tool could help veterinarians diagnose and respond to parvovirus outbreaks more effectively.
People also search for: puppy vomiting diarrhea parvovirus · how to test for canine parvovirus · rapid parvovirus test for dogs
Abstract
BACKGROUND: Canine parvovirus 2 (CPV-2) is one of the most common etiological agents that cause severe gastroenteritis in puppies. Early accurate diagnosis is important for infected dogs. In recent years, magnetic separation has become an efficient and useful tool for bioassays. In this study, polymerase chain reaction (PCR) combined with fluorescent lateral flow immunoassay (LFIA) based on magnetic purification assay was developed for the quantitative detection of CPV-2. RESULTS: The optimum working reaction volume and reaction time for LFIA was 100 μL and 2 min, respectively. The PCR-LFIA assay only detected CPV-2, and did not show cross-detection of non-CPV strains. Experiments showed analytical sensitivity of 3 × 10copies/μL and demonstrated the PCR-LFIA has a diagnostic agreement of 100% with conventional PCR on detection of clinical samples (22.6% positive, 14/62). Cutoff value is 146. The results were further verified by sequencing and BLAST software. The entire process from PCR step only takes ~ 80 min. CONCLUSIONS: This approach provides an attractive platform for rapid and quantitative detection of CPV-2, indicating great promise as a convenient molecular detection tool to facilitate disease outbreak investigations and response timely.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/30654823/