Porcine Cysticercosis: Possible Cross-Reactivity ofto GP50 Antigen in the Enzyme-Linked Immunoelectrotransfer Blot Assay.

Abstract

The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure toin pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, includingwhich have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6-8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulatingsp. antigens. From eight necropsied piglets; four were infected withmetacestodes whereas none had evidence ofinfection. Two resident dogs were subsequently confirmed to havetaeniasis. These results suggest GP50 cross-reactivity ininfected pigs, although controlled experimental infection is needed to confirm this hypothesis.