Preparation and mechanism study ofpolysaccharide-aluminum Pickering emulsion adjuvant for improving intestinal mucosal immune function.

Abstract

INTRODUCTION: Bovine viral diarrhea virus (BVDV) can induce diarrhea and mucosal tissue damage, and in severe cases, may result in fatal. Targeted adjuvants capable of enhancing intestinal IgA antibody responses represent an effective strategy for preventing bovine viral diarrhea. METHODS: In this study, anpolysaccharide Pickering emulsion (AHPPE) adjuvant was developed by incorporatinghoney polysaccharide (AHP) and retinoic acid (RA) with an aluminium-based adjuvant. Mice were subsequently immunized via intramuscular injection to evaluate the adjuvants' immunostimulatory potential. RESULTS: The results demonstrate that the particle size of AHPPE is 2133 nm, with excellent dispersion and stability. The encapsulation efficiencies for BVDV and AHP were 66.8% and 73.2%, respectively. AHPPE demonstrated recruitment of antigen-presenting cells at the injection site and activated IgA cells in the duodenum, jejunum, and ileum, inducing to increased IgA expression across multiple intestinal segments. Furthermore, AHPPE significantly induced serum IgG production and elevated levels of cytokines, including IL-4, IL-10, IL-17, IFN-&#x3b3;, and TNF-&#x3b1; (<0.05). Through sequencing analysis, it was found that IgA production may be induced via Intestinal immune network for IgA production, thereby mediating intestinal mucosal immune responses. CONCLUSION: Collectively, these findings indicate that AHPPE adjuvant administered via injection can simultaneously induce effective systemic immune and intestinal mucosal immunity. Therefore, as a novel intestinal-targeted adjuvant, AHPPE shows potential to enhance the specific intestinal mucosal immunity and systemic immunity of BVDV vaccine.