Presence and diversity ofisolated from layer farms in central Ecuador.

Abstract

Given the considerable role played byin the incidence of food contamination, around the world, surveillance of this infection is prioritized by both food producers and health care authorities. Data remains insufficient concerning the prevalence ofin poultry systems in Ecuador and in Latin America in general.In this study, we evaluated the presence and diversity ofserovars in samples taken from 21 layer farms and backyard layers in central Ecuador during August-November 2017.  was isolated following standardized methods (ISO 6579) and the serovar determination was carried out by PCR.A significant presence ofwas detected in the 21 farms evaluated, with a frequency of 76% (95% confidence interval (CI): 53-92) in environmental surfaces, 33% (95%CI: 15-57) in pooled cloacal swabs from layer hens, 33% (95% CI: 13-59) on feed samples, and 10% (95%CI: 1-30) in backyard layer feces from traditional local markets. The dominant serovars detected wereInfantis andTyphimurium.This study forms a basis for further surveillance ofserovars in layer farms in central Ecuador.