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Peer-reviewed veterinary case report

Pressure-inactivated FMDV: a potential vaccine.

Journal:
Vaccine
Year:
2004
Authors:
Ishimaru, Daniella et al.
Affiliation:
Departamento de Bioqu&#xed · Brazil
Species:
rabbit

Abstract

Foot-and-mouth disease virus (FMDV) is the causative agent of the foot-and-mouth disease (FMD). Alternative FMD vaccines have been pursued due to important disadvantages of the one currently in use. High hydrostatic pressure (HP) has been observed to inactivate some viruses. Here, we investigated the effects of HP on FMDV O1 Campos-Vallée (CVa) infectivity. A treatment consisting of 2.5 kbar at -15 degrees C and 1M urea, completely abolished FMDV infectivity, maintaining the integrity of its capsid structure. Moreover, its ability to elicit neutralizing antibody production in rabbits was preserved. Taken together, our results suggest that HP could be a safe, simple, cheap and reproducible way for viral vaccine production.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15149793/