Prevalence and molecular characterization of Giardia duodenalis in cattle and sheep from the Qinghai-Tibetan Plateau Area (QTPA), northwestern China.

Abstract

Giardia duodenalis is an important intestinal protozoan parasite with a wide range of hosts, including humans, livestock and wildlife. The purpose of this study was to determine the prevalence of G. duodenalis infections among cattle and sheep in the Qinghai-Tibetan Plateau Area (QTPA) and to assess the potential risk of the zoonotic transmission of this pathogen. A total of 454 stool specimens were collected and examined using the nested PCR method based on the G. duodenalis SSUrRNA gene fragment. Thirty-nine out of 389 cattle specimens examined were positive (10%) for the G. duodenalis infection. After the sequence analysis of the SSUrRNA gene, all detected G. duodenalis belong to assemblage E. No G. duodenalis infections were found in the 65 investigated samples from sheep. Our data therefore indicates that G. duodenalis is a common parasite in cattle in the QTPA, China and that cattle appear to be a reservoir of G. duodenalis for other animals and the environmental water supplies in the area.