Prevalence ofandgenes in coagulase negative staphylococci isolated from bovine mastitis in smallholder dairy farms in Turkey.

Abstract

This study aimed to investigate the presence ofandgenes in coagulase negative(CNS) species isolated from bovine mastitis in smallholder dairy farms by using PCR. A total of 602 mammary quarter milk samples belong to 170 cows with mastitis were used. Identification of species was achieved by using the commercial Gram-positive identification kit and a total of 52 (8.6%) CNS species were isolated. The most frequently isolated species was(n = 15, 28.8%), followed by(n = 12, 23.1%),(n = 8, 15.4%),(n = 5, 9.6%),(n = 4, 7.7%),(n = 4, 7.7%),(n = 2, 3.8%) and(n = 2, 3.8%). Thegene positivity was found in the 13 (25%) of strains. Of the strains carryinggene, eight also harbored thegene. A total ofgene positivity was found as 30.8% (n = 16) in 52 CNS species. In conclusion, the present study showed that CNS isolated from cows with mastitis may be reservoir ofandgenes. To our knowledge, this is the first study showing the presence ofandgenes in CNS species isolated from bovine with mastitis in the smallholder dairy farms in Turkey.