Peer-reviewed veterinary case report
Principles of bone marrow transplantation (BMT): providing optimal veterinary and husbandry care to irradiated mice in BMT studies.
- Journal:
- Journal of the American Association for Laboratory Animal Science : JAALAS
- Year:
- 2009
- Authors:
- Duran-Struuck, Raimon & Dysko, Robert C
- Affiliation:
- Massachusetts General Hospital · United States
- Species:
- rodent
Plain-English summary
Bone marrow transplantation (BMT) is a treatment often used for various types of cancers and certain metabolic diseases. This research focuses on how to properly care for mice that receive bone marrow transplants, especially since these mice are temporarily immunocompromised, meaning their immune systems are weakened. The study emphasizes the importance of keeping these mice in a clean environment to prevent infections, especially after they have been exposed to whole-body radiation, which is a common method used to prepare them for the transplant. It also highlights the need for careful oversight by animal care committees to ensure the well-being of the mice during this process. Overall, the research aims to improve the success of BMT by ensuring that the mice receive optimal care before, during, and after the procedure.
Abstract
Bone marrow transplantation (BMT) is the treatment of choice for many leukemias, solid tumors, and metabolic diseases. The field of bone marrow research is highly dependent on in vivo experimentation, because in vitro techniques do not mimic these complicated in vivo systems. Therefore, understanding the medical and husbandry care needs of these transiently immunodeficient bone marrow recipient animals is crucial for researchers, veterinary and animal care personnel. Here we discuss the principles of bone marrow transplantation, mouse pathogens that can interfere with transplantation research, and important husbandry and veterinary practices for mice that may help to minimize unnecessary infections during the transplantation process. Whole-body irradiation is one of the most common tools for myeloablation of the recipient's bone marrow. We discuss the crucial role of the irradiator for BMT research and the importance of aseptic husbandry practices to lessen the possibility of the irradiator for being a source for disease transmission. Finally, we discuss some important guidelines for Institutional Animal Use and Care Committees reviewing irradiation and BMT protocols.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19245745/