Pro-Inflammatory Cytokines in Aqueous Humor From ADAMTS10-Mutant Beagles at an Early Stage of Open-Angle Glaucoma.

Abstract

OBJECTIVE: To investigate the level of pro-inflammatory cytokines in aqueous humor from ADAMTS10-mutant research Beagles at an early stage of open-angle glaucoma (OAG). ANIMALS STUDIED: Twenty-four research Beagles were enrolled into this case-controlled study. Three groups of ADAMTS10-mutant research Beagles were enrolled in this study: (1) Intraocular pressure (IOP) 15-25&#x2009;mmHg (normotensive IOP), (2) IOP 25-30&#x2009;mmHg, and (3) IOP 30-36&#x2009;mmHg. An unaffected control group of Beagles that were either ADAMTS10-wildtype (WT) or heterozygote-carrier with normotensive IOPs were also enrolled. PROCEDURES: Aqueous humor samples were collected and analyzed for 16 pro-inflammatory cytokines using the Canine Cytokine SpikeMix and target mass spectrometry via multiple reaction monitoring (MRM-MS). Statistical differences between the four groups' pro-inflammatory cytokines, as well as correlations between pro-inflammatory cytokines and IOPs, were analyzed using Kruskal-Wallis tests and Spearman's rho (&#x3c1;) correlations, respectively. RESULTS: IOP 15-25&#x2009;mmHg (n&#x2009;=&#x2009;8); IOP 25-30&#x2009;mmHg (n&#x2009;=&#x2009;6); IOP 30-36&#x2009;mmHg (n&#x2009;=&#x2009;5); and Control (WT n&#x2009;=&#x2009;3, carrier n&#x2009;=&#x2009;2, total n&#x2009;=&#x2009;5). There were no differences between pro-inflammatory cytokines across the four groups (all p-values >&#x2009;0.05). IL-13 had a moderate positive correlation with IOP, but was non-significant (&#x3c1;&#x2009;=&#x2009;0.373, p&#x2009;=&#x2009;0.073); IL-1&#x3b2; had a moderate negative correlation with IOP but was also non-significant (&#x3c1;&#x2009;=&#x2009;-0.344, p&#x2009;=&#x2009;0.100). All other pro-inflammatory cytokines had only mild correlation with IOPs (|&#x3c1;|&#x2009;<&#x2009;0.229, p&#x2009;>&#x2009;0.05). CONCLUSIONS: There were no significant changes in the investigated pro-inflammatory cytokines with elevated IOP in canine ADAMTS10-OAG.