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Peer-reviewed veterinary case report

Production and purification of theVP1 capsid protein of a novel canine norovirus using the Saccharomyces cerevisiae expression system.

Journal:
Journal of microbiological methods
Year:
2012
Authors:
Pereira, Clara et al.
Affiliation:
REQUIMTE
Species:
dog

Abstract

The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8 mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/22985715/