Peer-reviewed veterinary case report
Production of equine herpesvirus 1 recombinant glycoprotein D and development of an agar gel immunodiffusion test for serological diagnosis.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Fuentealba, Nadia et al.
- Affiliation:
- Department of Virology
- Species:
- horse
Plain-English summary
Equine herpesvirus 1 and 4 are viruses that can make horses very sick. Researchers created a test using a part of the virus called glycoprotein D to help detect if a horse has been exposed to these viruses. This new test, called an agar gel immunodiffusion test, was found to be very accurate, correctly identifying almost all cases of infection. It is quick, affordable, and easy to use, making it a promising option for diagnosing these viral infections in horses. Overall, the test appears to be a reliable way to check for antibodies against equine herpesvirus 1 and 4.
Abstract
Equine herpesvirus 1 and 4 (EHV-1 and 4) infect most of the world's horses, causing serious clinical illness. Viral glycoproteins have been identified as the immunodominant antigens that generate the antiviral serological responses to EHV-1 and EHV-4 in infected horses. Here, glycoprotein D of EHV-1 was expressed by a recombinant baculovirus, purified and evaluated by a simple agar gel immunodiffusion test (AGID). Compared with virus neutralization, serological analysis by AGID showed good specificity (100%) and sensitivity (99.5%). The estimated Kappa values for repeatability and reproducibility were satisfactory. Thus, this rapid, inexpensive, simple and highly specific AGID test seems to be a valuable alternative tool for serological detection of antibodies against both EHV-1 and EHV-4.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24631347/