Peer-reviewed veterinary case report
Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay.
- Journal:
- Journal of microbiological methods
- Year:
- 2016
- Authors:
- Yeh, Hung-Yueh et al.
- Affiliation:
- United States Department of Agriculture · United States
Abstract
Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called "Simple Western" was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26784737/