Peer-reviewed veterinary case report
Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina.
- Journal:
- Parasitology research
- Year:
- 2011
- Authors:
- Zhao, Yuelan et al.
- Affiliation:
- College of Chinese Traditional Veterinary Science · China
Abstract
Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21499749/