Proposed genotype definition of Porcine sapelovirus.

Abstract

Conventionally, Porcine sapelovirus (PSV) has been considered to comprise a single geno- type, PSV-1; however, a potentially novel member of PSV was recently discovered. In the present study, we propose a genotype definition of PSV based on phylogenetic and genetic analyses of the polyprotein, P1, and VP1 genes of available PSV sequences. Two genotypes, with proposed names PSV-1 and PSV-2, were identified. Moreover, the cut-off values (number of differences per site between amino acid sequences) for the definition of genotypes were established to be 0.1115 (polyprotein), 0.176 (P1), and 0.272 (VP1). The findings of this study are expected to enrich knowledge of PSV classification.