Peer-reviewed veterinary case report
Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus.
- Journal:
- Veterinary immunology and immunopathology
- Year:
- 2015
- Authors:
- Zhu, Yingqi et al.
- Affiliation:
- Shanghai Veterinary Research Institute · China
Abstract
A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV). The sigma C gene of NDRV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. After transfected into BHK-21 cells, the antigenicity of the sigma C protein was confirmed using an indirect immunofluorescence and Western blot assay. Immunogenicity was studied in ducklings. The results showed that NDRV-specific antibodies, neutralizing antibodies, IFN-γ and IL-4 were well induced in ducklings. Furthermore, all of the ducklings were protected against challenge with wild-type NDRV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25863968/