Peer-reviewed veterinary case report
Protein differences in lung fluid of West Highland White Terriers
By Lilja-Maula, Liisa I O et al.·Published in American journal of veterinary research·2013·Department of Equine and Small Animal Medicine·View original on PubMed →
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Original publication title: Proteomic analysis of bronchoalveolar lavage fluid samples obtained from West Highland White Terriers with idiopathic pulmonary fibrosis, dogs with chronic bronchitis, and healthy dogs.
- Species:
- dog
Plain-English summary
A group of West Highland White Terriers with idiopathic pulmonary fibrosis (IPF) had samples taken from their lungs to study the proteins present in their bronchoalveolar lavage fluid. The researchers found that certain proteins were more abundant in the dogs with IPF compared to healthy dogs, indicating a possible response to lung disease. However, they did not find specific markers that could clearly distinguish IPF from chronic bronchitis. This research helps in understanding the underlying mechanisms of these lung diseases in dogs, but more work is needed to identify clear biomarkers for IPF.
People also search for: West Highland White Terrier lung disease · idiopathic pulmonary fibrosis in dogs · dog chronic bronchitis symptoms
Abstract
OBJECTIVE: To evaluate protein expression in bronchoalveolar lavage fluid (BALF) obtained from West Highland White Terriers with idiopathic pulmonary fibrosis (IPF), dogs with chronic bronchitis, and healthy control dogs to identify potential biomarkers for IPF. SAMPLES: BALF samples obtained from 6 West Highland White Terriers with histologically confirmed IPF, 5 dogs with chronic bronchitis, and 4 healthy Beagles. PROCEDURES: Equal amounts of proteins in concentrated BALF samples were separated via 2-D differential gel electrophoresis. Proteins that were differentially expressed relative to results for healthy control dogs were identified with mass spectrometry and further verified via western blotting. RESULTS: Expression of 6 proteins was upregulated and that of 1 protein was downregulated in dogs with IPF or chronic bronchitis, compared with results for healthy dogs. Expression of proteins β-actin, complement C3, α-1-antitrypsin, apolipoprotein A-1, haptoglobin, and transketolase was upregulated, whereas expression of lysozyme C was downregulated. CONCLUSIONS AND CLINICAL RELEVANCE: Proteomics can be used to search for biomarkers and to reveal disease-specific mechanisms. The quantitative comparison of proteomes for BALF obtained from dogs with IPF and chronic bronchitis and healthy dogs revealed similar changes for the dogs with IPF and chronic bronchitis, which suggested a common response to disease processes in otherwise different lung diseases. Specific biomarkers for IPF were not identified.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23270360/