Proteomics Analysis ofMetacestode Stage.

Abstract

() is one of the most robust of tapeworm parasites that is widely distributed around the world. Information of proteins ofhas not previously been reported. Using liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis, the proteome ofmetacestode was profiled and a total of 408 proteins were identified. Of these, 26.5% (108/408) were annotated to be associated with metabolic pathways. Consistently, Gene Ontology analysis showed that those identified proteins were mainly classified into metabolic process of the biological processes. A set of metabolic enzymes, including Fructose-1,6-bisphosphate aldolase, enolase, Glucan phosphorylase, and phosphoenolpyruvate carboxykinase, were abundant inmetacestodes. In addition, some rare but interesting proteins like antigens (such as tegument protein and Antigen B) were identified. The two recombinant proteins of tegument protein and Antigen B were well-recognized by the sera from theinfected mice. Themetacestode proteome might help to find new candidates for the immunodiagnosis and vaccine development and provide valuable information onbiology.