Peer-reviewed veterinary case report
Protocol for analyzing the microstructure of nuclear fiber layers by using structured illumination microscopy coupled with Arivis 4D.
- Year:
- 2026
- Authors:
- Guan M et al.
- Affiliation:
- The Interdisciplinary Research Group on Microscopic Imaging and Drug Development · China
Abstract
Here, we present a protocol for quantitatively assessing the microstructure of nuclear fiber layers by immunolabeling of a nuclear skeleton protein lamin A through structured illumination microscopy (SIM) coupled with an innovative algorithm in Arivis 4D. We describe steps of sample preprocessing for SIM and imaging of the inner-nucleus skeleton network of lamin A. We then detail procedures for creating a novel algorithm in Arivis 4D software to quantitatively assess the microparameters of the nuclear skeleton in a high-resolution image.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://europepmc.org/article/MED/41477760