Peer-reviewed veterinary case report
Protocol for chemogenetic activation of basal ganglia D1-MSNs and behavioral assessments in a primate Parkinson's disease model.
- Journal:
- STAR protocols
- Year:
- 2024
- Authors:
- Chen, Yefei et al.
- Affiliation:
- Department of Anesthesiology · China
Abstract
A circuit-based gene therapy strategy for Parkinson's disease (PD) has been shown to significantly reverse core symptoms in both murine and primate PD models. Here, we present a comprehensive workflow to specifically manipulate dopamine receptor D-expressing medium spiny neurons by retrograde adeno-associated virus (AAV) transduction and chemogenetic activation using a designer toolkit. We describe steps for AAV injections and PD primate model induction. We then detail behavioral measurements to assess the therapeutic efficacy of the therapy for motor symptoms.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/39644495/