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Peer-reviewed veterinary case report

Protocol for establishing a mouse model of bilateral maxillary first molar extraction to study alveolar bone healing.

Journal:
STAR protocols
Year:
2026
Authors:
Jiang, Jiarui et al.
Affiliation:
School of Stomatology · China

Abstract

Alveolar bone healing following tooth extraction is a complex biological process. Here, we present a protocol for establishing a reproducible mouse model of bilateral maxillary first molar extraction to study alveolar bone healing. We describe steps for surgery, postoperative care, and tissue collection for downstream genomic, histological, and micro-computed tomography (CT) analyses. We provide practical strategies to minimize root fracture and soft tissue injury, ensuring procedural consistency and high-quality outcomes.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/41701575/