Peer-reviewed veterinary case report
Protocol for evaluating CD8T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy.
- Journal:
- STAR protocols
- Year:
- 2022
- Authors:
- Sun, Yuting et al.
- Affiliation:
- Institute of Laboratory Animal Science · China
Abstract
Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIV-infected rhesus macaques with an HIV fusion-inhibitory lipopeptide (LP-98) monotherapy. We also present the steps for adoptive transfer of an anti-CD8 antibody into a stable virologic control (SVC) group of LP-98-treated monkeys, confirming a direct role of CD8T cells in SVC macaques. For complete details on the use and execution of this protocol, please refer to Xue et al. (2022).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35776642/