Peer-reviewed veterinary case report
Protocol for fabrication and utilization of the device for axon-cancer cell interaction testing.
- Year:
- 2026
- Authors:
- Velazquez-Quesada I et al.
- Affiliation:
- Bioengineering Department · United States
Abstract
The device for axon and cancer cell interaction testing (DACIT) mimics neuron-cancer interactions by compartmentalizing neuron soma and axons. Here, we present a protocol to design, fabricate, load, and image the DACIT. We describe steps for DACIT master generation, smooth-on mold replication, and DACIT fabrication. We then detail procedures for cell loading in 2D and 3D settings. This protocol also includes custom 3D-printed imaging holders. For complete details on the use and execution of this protocol, please refer to Velazquez-Quesada et al.<sup>1</sup>.
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Search related cases →Original publication: https://europepmc.org/article/MED/41468264