Peer-reviewed veterinary case report
Protocol for inducing severe Scn2a insufficiency in mice by intracerebroventricular antisense oligonucleotide injection.
- Journal:
- STAR protocols
- Year:
- 2024
- Authors:
- Li, Melody & Kuhn, Bernd
- Affiliation:
- Okinawa Institute of Science and Technology Graduate University (OIST) · Japan
- Species:
- rodent
Abstract
SCN2A loss-of-function variants cause a range of neurodevelopmental disorders. Here, we present a protocol to induce severe Scn2a insufficiency in mice. We describe steps for intracerebroventricular (ICV) antisense oligonucleotide (ASO) injection that causes a selective downregulation of Scn2a and ASO-mediated mRNA degradation. We then detail procedures for qPCR and western blot protocol to measure Scn2a mRNA and protein. This protocol can be used as a mouse model for behavioral and in vivo two-photon Caimaging.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/38796847/