Peer-reviewed veterinary case report
Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans.
- Year:
- 2025
- Authors:
- Osborne OM et al.
- Affiliation:
- University of Miami Miller School of Medicine · United States
- Species:
- rodent
Abstract
Here, we present a protocol for isolating microvessels from fresh or snap-frozen brain tissue from mice and humans, followed by visualization of RNA utilizing RNAscope hybridization for quantification of mRNA. We describe the steps for sample preparation and isolation, fixation, and hybridization. This protocol was specifically designed to integrate with RNAscope in situ hybridization. Although the protocol was developed in a mouse model, it can be optimized for use in other organisms, including human brain samples.
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Search related cases →Original publication: https://europepmc.org/article/MED/39772387