Peer-reviewed veterinary case report
How to image and analyze Hibiscus trionum petal patterns
By Riglet L & Moyroud E.·2026·University of Cambridge, United Kingdom·View original on Europe PMC →
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Original publication title: Protocol for the quantitative imaging and segmentation of spatial patterns in Hibiscus trionum petals.
Plain-English summary
This research focuses on improving how we study the growth patterns of flower petals, specifically in the Hibiscus trionum plant. The authors have developed a detailed method that includes steps for preparing the flower buds, staining the petals to make them easier to see, and using special imaging techniques to analyze the petal surface. They also explain how to combine images and process them using specific software to better understand the cells in the petals. This protocol aims to help scientists explore the diversity of flower shapes more effectively.
Abstract
Most developmental processes underpinning the diversity of floral forms remain unexplored due to limitations in current imaging approaches. Here, we present a protocol to spatially analyze tissue-wide cell growth dynamics through confocal imaging and segmentation of the adaxial petal epidermis in Hibiscus trionum. We describe steps for dissecting buds, staining petals with a membrane dye, and acquiring images suitable for epidermal reconstruction. We detail procedures for image stitching and processing in ImageJ and for surface extraction and cell segmentation in MorphoGraphX. For complete details on the use and execution of this protocol, please refer to Riglet et al.<sup>1</sup>.
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Search related cases →Original publication on Europe PMC: https://europepmc.org/article/MED/41642711