Peer-reviewed veterinary case report
Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.
- Journal:
- STAR protocols
- Year:
- 2024
- Authors:
- Colenbier, Robin et al.
- Affiliation:
- Faculty of Medicine and Health Sciences
Abstract
The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/38492226/