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Peer-reviewed veterinary case report

Protocol to establish a stable_AML mouse model.

Journal:
STAR protocols
Year:
2022
Authors:
Lu, Jun et al.
Affiliation:
Department of Pharmacology and Bone Marrow Transplantation Center of the First Affiliated Hospital · China

Abstract

Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse_AML model to investigate AML. We have optimized the protocols for retrovirus infection, bone marrow transplantation (BMT), and leukemia monitoring to create a stable mouse model. In particular, we have used two rounds of BMT to enhance stability and efficiency. This model can be used to conduct drug administration and/or other interventions easily. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2022).

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Original publication: https://pubmed.ncbi.nlm.nih.gov/35874469/