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Peer-reviewed veterinary case report

Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2015
Authors:
Kint, Joeri et al.
Affiliation:
Wageningen Institute of Animal Sciences · Netherlands
Species:
bird

Abstract

Quantification of the number of infectious viruses in a sample is a basic virological technique. In this chapter we provide a detailed description of three techniques to estimate the number of viable infectious avian coronaviruses in a sample. All three techniques are serial dilution assays, better known as titrations.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/25720474/