Peer-reviewed veterinary case report
Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2021
- Authors:
- Nilsson, Per H et al.
- Affiliation:
- Department of Immunology
Abstract
Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33847930/