Peer-reviewed veterinary case report
Detecting Bordetella bronchiseptica in dog lung fluid with PCR
By Canonne, A M et al.·Published in Journal of veterinary internal medicine·2016·Faculty of Veterinary Medicine·View original on PubMed →
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Original publication title: Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella bronchiseptica Infection.
- Species:
- dog
Plain-English summary
A group of 24 dogs with a Bordetella bronchiseptica infection, which can cause coughing and breathing problems, were tested to see how well different methods could detect the bacteria. Researchers found that a test called quantitative PCR (qPCR) was very effective, identifying the bacteria in nearly all the affected dogs. Additionally, they observed bacteria sticking to the cells in the lung fluid samples, which helped confirm the diagnosis. This study suggests that using qPCR and examining lung fluid can quickly and accurately diagnose this infection in dogs, leading to better treatment outcomes.
People also search for: dog coughing Bordetella treatment · how to diagnose dog bronchitis · Bordetella infection symptoms in dogs
Abstract
BACKGROUND: The use of quantitative PCR (qPCR) for detection of Bordetella bronchiseptica in bronchoalveolar lavage fluid (BALF) and demonstration of bacteria adhering to ciliated epithelial cells in BALF or bronchial brushing fluid (BBF) has not been assessed in a series of affected dogs. Coinfections can worsen the clinical severity in bordetellosis, but the specific association with Mycoplasma cynos has not been evaluated. OBJECTIVES: To assess the utility of culture, qPCR and cytologic examination of cytospin preparations in the diagnosis of bordetellosis in dogs and the influence of coinfection by M. cynos on disease severity. ANIMALS: Twenty-four referred dogs with B. bronchiseptica infection and 10 healthy dogs. METHODS: Retrospective case series. qPCR (B. bronchiseptica and M. cynos) and culture results from BALF were recorded. Cytospin preparations from BALF and BBF were reviewed. qPCR on BALF from 10 healthy dogs were used as negative control. RESULTS: The BALF culture and qPCR detected B. bronchiseptica in 14/24 and 18/18 dogs, respectively. Coccobacilli were found adhering to ciliated epithelial cells in 20 of the 21 BALF cytologic preparations where epithelial cells were found, and 2/3 BBF cytologic preparations. Quantitative PCR detected a low level of B. bronchiseptica in one healthy dog. The frequency of detection of M. cynos was not significantly different in B. bronchiseptica (9/17 dogs) compared with healthy dogs (2/10 dogs) (P = .09). CONCLUSION AND CLINICAL IMPORTANCE: Quantitative PCR detection of B. bronchiseptica in BALF appears to be a useful diagnostic tool. Cytologic examination of BALF or BBF, when positive, allows a rapid and reliable diagnosis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/27461723/