Peer-reviewed veterinary case report
Protein changes in lung fluid of West Highland terriers with lung
By Maher, Rosemary E et al.·Published in BMC veterinary research·2022·Institute of Systems and Integrative Biology, United Kingdom·View original on PubMed →
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Original publication title: Quantitative proteomic analysis of bronchoalveolar lavage fluid in West Highland white terriers with canine idiopathic pulmonary fibrosis.
- Species:
- dog
Plain-English summary
A 7-year-old West Highland White Terrier was diagnosed with canine idiopathic pulmonary fibrosis (CIPF), a serious lung disease that causes coughing, difficulty breathing, and exercise intolerance. Unfortunately, there is no cure for this condition, and it often leads to respiratory failure. Researchers studied the dog's lung fluid to identify proteins that could help differentiate between healthy dogs and those with CIPF. They found several proteins linked to inflammation and fibrosis that were more abundant in the affected dogs, but they did not find any markers indicating that stomach contents were causing the lung issues. Understanding these protein changes may help in evaluating future treatment options for dogs with CIPF.
People also search for: West Highland White Terrier coughing · canine idiopathic pulmonary fibrosis treatment · dog breathing problems · lung disease in dogs · dog exercise intolerance symptoms
Abstract
BACKGROUND: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white terriers (WHWTs), lacks curable treatment and has a poor prognosis. Aspiration of gastroesophageal refluxate may play a role in the development of CIPF. In the first part of this study, we completed label-free quantitative proteomic analysis of bronchoalveolar lavage fluid (BALF) from CIPF and healthy WHWTs. In the second part, we evaluated potential protein markers of reflux aspiration from canine gastric juice and vomitus and whether these were present in BALF from the two groups. RESULTS: Across all BALF samples, 417 proteins were identified, and of these, 265 proteins were identified by two or more unique tryptic peptides. Using the 265 high confidence assignments, the quantitative proteome profiles were very similar in the two cohorts, but they could be readily resolved by principal component analysis on the basis of differential protein expression. Of the proteins that were differentially abundant in the two groups, several (including inflammatory and fibrotic markers) were elevated in CIPF, and a smaller, more diverse group of proteins were diminished in CIPF. No protein markers indicative of reflux aspiration were identified. CONCLUSIONS: Label-free proteomics allowed discrimination between CIPF and healthy WHWTs, consistent with fibrotic process but did not provide clear evidence for gastrointestinal aspiration. The measurement of proteins may provide a proteomics signature of CIPF that could be used to evaluate treatment options.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/35354473/