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Peer-reviewed veterinary case report

Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds

Journal:
Italian Journal of Animal Science
Year:
2010
Authors:
M. Cagnazzo et al.

Abstract

The quantitative real time-PCR (QRT-PCR) is a very sensitive method used to quantify mRNA level in gene expression analysis. Combining amplification, detection and quantification in a single step, allows a more accurate measurement compared to the traditional PCR end point analysis (Pfaffl, 2001; Bustin, 2002).

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Original publication: https://doi.org/10.4081/ijas.2005.2s.91