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Peer-reviewed veterinary case report

RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African swine fever virus DNA.

Journal:
Chemical communications (Cambridge, England)
Year:
2026
Authors:
Wu, Hongyu et al.
Affiliation:
Institute of Advanced Materials (IAM) · China

Abstract

An RAA-CRISPR-HCR (RCH) cascade amplification system was developed, which generates an ultrasensitive colorimetric response to low concentrations of African swine fever virus (ASFV) DNA with a detection limit of 1 copy µL. It demonstrates excellent accuracy and specificity in real samples, facilitating efficient on-site visual early detection of ASFV.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/41645777/