Peer-reviewed veterinary case report
Fast and sensitive PCR test detects feline immunodeficiency virus
By Wilkes, Rebecca Penrose et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2015·Department of Biomedical and Diagnostic Sciences, United States·View original on PubMed →
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Original publication title: Rapid and sensitive detection of Feline immunodeficiency virus using an insulated isothermal PCR-based assay with a point-of-need PCR detection platform.
- Species:
- cat
Plain-English summary
A new test for Feline Immunodeficiency Virus (FIV) was developed to help quickly diagnose infected cats. This test can provide results in about an hour and is designed to be used outside of a traditional lab setting. In a study with 76 cat samples, the test was highly accurate, detecting 97.78% of positive cases and correctly identifying all healthy cats. This means that if your cat is showing signs of illness related to FIV, this new test could help your veterinarian diagnose the virus quickly and accurately, leading to better management of your cat's health.
People also search for: cat FIV symptoms · how to test for FIV in cats · FIV treatment options for cats
Abstract
Feline immunodeficiency virus (FIV) is an important infectious agent of cats. Clinical syndromes resulting from FIV infection include immunodeficiency, opportunistic infections, and neoplasia. In our study, a 5' long terminal repeat/gag region-based reverse transcription insulated isothermal polymerase chain reaction (RT-iiPCR) was developed to amplify all known FIV strains to facilitate point-of-need FIV diagnosis. The RT-iiPCR method was applied in a point-of-need PCR detection platform--a field-deployable device capable of generating automatically interpreted RT-iiPCR results from nucleic acids within 1 hr. Limit of detection 95% of FIV RT-iiPCR was calculated to be 95 copies standard in vitro transcription RNA per reaction. Endpoint dilution studies with serial dilutions of an ATCC FIV type strain showed that the sensitivity of lyophilized FIV RT-iiPCR reagent was comparable to that of a reference nested PCR. The established reaction did not amplify any nontargeted feline pathogens, including Felid herpesvirus 1, feline coronavirus, Feline calicivirus, Feline leukemia virus, Mycoplasma haemofelis, and Chlamydophila felis. Based on analysis of 76 clinical samples (including blood and bone marrow) with the FIV RT-iiPCR, test sensitivity was 97.78% (44/45), specificity was 100.00% (31/31), and agreement was 98.65% (75/76), determined against a reference nested-PCR assay. A kappa value of 0.97 indicated excellent correlation between these 2 methods. The lyophilized FIV RT-iiPCR reagent, deployed on a user-friendly portable device, has potential utility for rapid and easy point-of-need detection of FIV in cats.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/26185125/