Peer-reviewed veterinary case report
Rapid detection of bovine herpesvirus 1 in bovine semen by loop-mediated isothermal amplification (LAMP) assay.
- Journal:
- Archives of virology
- Year:
- 2014
- Authors:
- Pawar, Sachin S et al.
Plain-English summary
Bovine herpesvirus 1 (BoHV-1) is a common virus that can cause reproductive problems in cattle, which can lead to financial losses for farmers. Researchers developed a new test called a loop-mediated isothermal amplification (LAMP) assay to quickly detect this virus in bull semen. This test can provide results in just 90 minutes and is very sensitive, able to detect tiny amounts of the virus. When tested on 118 semen samples, the LAMP assay showed high accuracy, correctly identifying 97% of positive cases and 100% of negative cases compared to another testing method. Overall, this new LAMP assay is a fast, reliable, and cost-effective way to check for BoHV-1 in bull semen.
Abstract
Bovine herpesvirus 1 (BoHV-1) is the most common viral pathogen found in bovine semen, causing numerous reproductive disorders leading to economic losses to the cattle industry. For rapid detection of BoHV-1 in bovine semen, in this study, we applied a loop-mediated isothermal amplification (LAMP) assay. The assay could be completed within 90 min, including total DNA isolation, target amplification, and visual interpretation of positive or negative results with the naked eye. The assay detected as little as 10 fg of BoHV-1 DNA per reaction. The analytical sensitivity of the assay was 0.2 TCID50 BoHV-1 per reaction, which was 100 times more sensitive than conventional PCR and comparable to TaqMan real-time PCR. The applicability of the assay was assessed by analysing 118 semen samples collected from breeding bulls. On comparison with TaqMan real-time PCR, the LAMP assay had a diagnostic sensitivity of 97 %, specificity of 100 %, and accuracy of 99.2 % for detection of BoHV-1 in bovine semen. The LAMP assay developed in this study is a rapid, sensitive, and cost-effective alternative for detection of BoHV-1 in bovine semen.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24122110/