Peer-reviewed veterinary case report
How to quickly test for foot-and-mouth disease, flu, and swine fever
By Wernike, Kerstin et al.·Published in Journal of virological methods·2013·Institute of Diagnostic Virology, Germany·View original on PubMed →
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Original publication title: Rapid detection of foot-and-mouth disease virus, influenza A virus and classical swine fever virus by high-speed real-time RT-PCR.
Plain-English summary
Researchers have developed a fast and sensitive testing method to quickly detect three serious animal diseases: foot-and-mouth disease, influenza A, and classical swine fever. This new test can provide results in under 28 minutes, making it very useful during outbreaks or for monitoring these diseases. The tests were validated using samples from infected animals and showed reliable results across different testing machines. This rapid testing method could be a great tool for veterinarians to help control and monitor these contagious viruses in animals.
Abstract
High sensitivity, minor risk of cross-contamination and in particular the rapid reaction time make quantitative real-time polymerase chain reaction (qPCR) assays well suited for outbreak investigations as well as for monitoring epidemics of pathogens. In this study qPCR assays for three highly contagious animal diseases, namely foot-and-mouth-disease (FMD), influenza A (IA) and classical swine fever (CSF) have been developed. Furthermore, an amplification control targeting 18S ribosomal RNA was included. Each assay was validated with samples from infected animals using three different standard qPCR-machines in two thermal profiles: one standard and one high-speed approach, respectively. The high-speed PCR assays allowed the reliable diagnosis of FMD, influenza A and CSF in less than 28 min with an analytical sensitivity of at least 200 genome copies/μl in every case, with slight differences regarding reaction time and sensitivity for the individual PCR-cycler instruments. Therefore, the newly established rapid RT-PCR systems will be a valuable method for the monitoring and control of these three important viruses and will be a robust option for the development of novel molecular pen-side tests.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23702025/