Peer-reviewed veterinary case report
Rapid detection of Tembusu virus by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP).
- Journal:
- Transboundary and emerging diseases
- Year:
- 2012
- Authors:
- Tang, Y et al.
- Affiliation:
- Zoology Institute · China
- Species:
- bird
Abstract
A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of Tembusu virus (TMUV) infection. The reaction was performed in one step in a single tube at 64°C for 45 min, with SYBR Green I dye added prior to amplification. The detection limit of the RT-LAMP assay was approximately 10 copies/μl, and no cross-reaction with other avian viruses was observed. The assay was evaluated further for the diagnosis of TMUV in field samples and compared with conventional RT-PCR, demonstrating that results of the RT-LAMP assay corresponded to those of conventional RT-PCR. In conclusion, RT-LAMP with SYBR Green I dye was shown to be a sensitive, simple assay for the rapid diagnosis of TMUV infection in ducks.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/21914154/